Amnion Cells in Tailored Hydrogels Deposit Human Amnion Native Extracellular Matrix

Fetal therapies regularly result in iatrogenic preterm premature rupture of the fetal membranes (iPPROM), which is associated with birth. Biomaterials that promote healing traumatized (FMs) may prevent iPPROM-associated births, addressing this unmet clinical need. Here, a fully defined synthetic poly(ethylene glycol) (PEG) hydrogel developed to study functions human amnion-derived mesenchymal stromal cells (hAMCs) 3D cultures. A pipeline analyze extracellular matrix (ECM) proteins deposited by hAMCs PEG hydrogels established involving label-free quantification mass-spectrometry data. Owing contaminant-free and short bovine serum (FBS)-free culture period, 128 ECM proteins, 97 are present native amnion, identified. Upon stimulation platelet-derived growth factor BB (PDGF-BB), cell proliferation migration inducing factor, remodel their surroundings deposit pericellularly. Among most abundantly amnion transforming β-induced protein ig-h3 (TGFβi), very distinctive involved wound cascade, These data support potential PDGF-BB repair FMs encourage its use for engineering biomaterials FM healing, ultimately iPPROM.